IJMST 2017 Volume 7 Issue 1

International Journal of Medical Sciences and Technology (IJMST) ISSN:0974-5343

An Open Access Journal -- NO Fees -- NO Processing Charges -- 100% Non Profit Initiatives


Assessment of Inter-alpha-trypsin heavy chain-4 peptides in differential diagnosis of ischemic and hemorrhagic stroke. Neha H. Lande, Amit R. Nayak, Anuja P. Kawle, Dinesh P. Kabra , Nitin Chandak, Lokendra Singh, Hatim F. Daginawala, Rajpal S. Kashyap. IJMST (2017), 7(1):1-9



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Title:
Assessment of Inter-alpha-trypsin heavy chain-4 peptides in differential diagnosis of ischemic and hemorrhagic stroke

Authors & Affiliation:
Neha H. Lande, Amit R. Nayak, Anuja P. Kawle, Dinesh P. Kabra , Nitin Chandak, Lokendra Singh, Hatim F. Daginawala, Rajpal S. Kashyap
Biochemistry Research Centre, Central India Institute of Medical Sciences, 88/2 Bajaj Nagar, Nagpur-440010, Maharashtra, INDIA
nehalande89@gmail.com, amit_ciims@rediffmail.com, anujryankawle@gmail.com, dineshkabra@gmail.com, ch_nitin26@yahoo.co.in, ciimsresearchlab@gmail.com, hfd_ciims@rediffmail.com, raj_ciims@rediffmail.com

Abstract:
Background: Differential diagnosis of Ischemic stroke (IS) and Hemorrhagic Stroke (HS) patient is crucial for augmenting the specific treatment. Objective: In the present study, we aimed to evaluate the role of the Inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4) for the differential diagnosis of IS and HS patient. Material and method: This prospective study was carried out on 104 IS and 15 HS patients. Blood samples were collected at admission. Serum level of ITIH4 was estimated by indirect ELISA method using anti-ITIH4 peptide antibody (anti-ITIH4-peptide 1 to 9). Diagnosis sensitivity and specificity were determined by ROC curve analysis. Results: Out of panel of 9 antibodies, expression of Anti-ITIH4 peptide antibody 2 (p=0. 04), 5 (p=0.001), & 6 (p=0.0005) were significantly different between AIS and HS. Results shows sensitivity of  100%, 94%,  100%  and specificity of 50% , 52% and 61% by anti-ITIH4 peptide antibody 2 (Cut off- >0.764; AUC- 0.720 ; 95% Cl 0.633-0.796), 5(Cut off- >0.774; AUC-0.779;  95% Cl 0.696-0.848) & 6  (Cut off- >0.738; AUC- 0.816; 95%Cl 0.737-0.879) respectively for differential diagnosis of AIS and HS.. Conclusion: Present study suggest that the Anti- ITIH4-peptide 2, 5, and 6 may be useful for differential diagnosis of IS and HS.

Key words:
Acute Ischemic Stroke, Hemorrhagic Stroke, differential diagnosis, blood biomarkers



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Prof. Dr. Prabhu Britto Albert,
Jan 31, 2017, 10:08 PM
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Prof. Dr. Prabhu Britto Albert,
Jan 31, 2017, 10:07 PM